'Calculate average pixel intensity for each frame of tif movie
I imported a tif movie into python which has the dimensions (150,512,512). I would like to calculate the mean pixel intensity for each of the 150 frames and then plot it over time. I could figure out how to calculate the mean intensity over the whole stack (see below), but I am struggling to calculate it for each frame individually.
from skimage import io
im1 = io.imread('movie.tif')
print("The mean of the TIFF stack is:")
print(im1.mean())
How can I get the mean pixel intensities for each frame?
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