tkinter matplotlib error _tkinter.TclError: image "pyimage1" doesn't exist in Pycharm

Question

I am trying to run following code, which run successfully... but when I am trying to import matplotlib it give error ----> _tkinter.TclError: image "pyimage1" doesn't exist while using PyCharm, but with python IDLE it works without error.

import cv2
from tkinter import *
from PIL import Image, ImageTk
import datetime
import openpyxl
#from matplotlib import pyplot as plt

def show_frames():
    im = cap.read()[1]
    Image1= cv2.cvtColor(im,cv2.COLOR_BGR2RGB)
    img = Image.fromarray(Image1)
    imgtk = ImageTk.PhotoImage(image = img)
    label.imgtk = imgtk
    label.configure(image=imgtk)
    label.after(20, show_frames)

def capture():
    I = cap.read()[1]
    save_name = str(datetime.datetime.now().today()).replace(":", " ") + ".jpg"
    cv2.imwrite(save_name, I)


 #here creating window and GUI
 win = Tk()
 win.geometry("1200x650")
 win.title('Portable Optical Spectrometer')

 L2 = Label(win,text = " Camera  ",font=("times new roman",20,"bold"),bg="white",fg="red").grid(row=0, column=0)
 L2 = Label(win,text = " Spectrum Graph ",font=("times new roman",20,"bold"),bg="white",fg="red").grid(row=0, column=2)
 label =Label(win)
 label.grid(row=1, column=0)
 cap = cv2.VideoCapture(0)

 show_frames()
 B1 = Button(win,text="Capture",font=("Times new roman",20,"bold"),bg="white",fg="red",command=capture()).grid(row=3, column=0, )
 B1 = Button(win,text="Analysis",font=("Times new roman",20,"bold"),bg="white",fg="red",).grid(row=4, column=0)

 L1 = Label(win,text = "Detected Material is:  ",font=("times new roman",20,"bold"),bg="white",fg="red").grid(row=4, column=1)
 Output = Text(win, height = 3,width = 25,bg = "light cyan").grid(row=4, column=2)


 win.mainloop()
 cap.release()

can someone suggest how to solve this error