'Python subprocess returning exception

I have the following code that has been working until recently:

        si = STARTUPINFO()
        si.dwFlags |= STARTF_USESHOWWINDOW
        env = environ
        self.popen = Popen('python', [path + '\opt_module_v3.py'] + vlist, stdout=PIPE, stderr=STDOUT, stdin=PIPE, universal_newlines=True, bufsize=1, startupinfo=si, env=env)
        lines_iterator = iter(self.popen.stdout.readline, b"")

it calls the opt_module_v3.py and returns messages as the child process runs. When I run the main code, I'm getting the following exception:

   self.popen = Popen('python', [path + '\opt_module_v3.py'] + vlist, stdout=PIPE, 
stderr=STDOUT, stdin=PIPE, universal_newlines=True, bufsize=1, startupinfo=si, env=env)
File "C:\ProgramData\Anaconda3\lib\site-packages\spyder_kernels\customize\spydercustomize.py", 
line 108, in __init__
super(SubprocessPopen, self).__init__(*args, **kwargs)
TypeError: __init__() got multiple values for argument 'bufsize'

Like I mentioned, this code was working before but I might have stuffed up since I've been working on improvements. Any clues on what could be happening here?

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'Python subprocess returning exception

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