Pandas return column data as list without duplicates

Question

This is just an oversimplification but I have this large categorical data.

Name   Age Gender 
John    12 Male 
Ana     24 Female
Dave    16 Female
Cynthia 17 Non-Binary
Wayne   26 Male
Hebrew  29 Non-Binary

Suppose that it is assigned as df and I want it to return as a list with non-duplicate values:

'Male','Female','Non-Binary'

I tried it with this code, but this returns the gender with duplicates

list(df['Gender'])

How can I code it in pandas so that it can return values without duplicates?