OpenCV - Determine detected objects angle from "center" of frame

Question

I am working on a machine vision project and need to determine the angle of an object in x and y relative to the center of the frame (center in my mind being where the camera is pointed). I originally did NOT do a camera calibration (calculated angle per pixel by taking a picture of a dense grid and doing some simple math). While doing some object tracking I was noticing some strange behaviour which I suspected was due to some distortion. I also noticed that an object that should be dead center of my frame was not, the camera had to be shifted or the angle changed for that to be true.

I performed a calibration in OpenCV and got a principal point of (363.31, 247.61) with a resolution of 640x480. The angle per pixel obtained by cv2.calibrationMatrixVales() was very close to what I had calculated, but up to this point I was assuming center of the frame was based on 640/2, 480/2. I'm hoping that someone can confirm, but going forward do I assume that my (0,0) in cartesian coordinates is now at the principal point? Perhaps I can use my new camera matrix to correct the image so my original assumption is true? Or I am out to lunch and need some direction on how to achieve this.

Also was my assumption of 640/2 correct or should it technically have been (640-1)/2. Thanks all!