'perMANOVA for small sample size

I have data of 6 groups with sample size of n = 2, 10, 2, 9, 3, 1 and I want to perform Permutational multivariate analysis of variance (PERMANOVA) on these data.

My question is: Is it correct to run perMANOVA on these data with the small sample size? The results look strange for me because the group of n = 1 showed insignificant difference to other groups although the graphical representation of the groups clearly show a difference.

Thank you



Solution 1:[1]

I would not trust any result with group of n=1 because there is no source of variation to define difference among groups.

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Solution Source
Solution 1 Mauricio Camargo