How to use a database connection string in App.config?

Question

I use EF 6, and if I use a hardcode database connection in the OnConfiguring:

protected override void OnConfiguring(DbContextOptionsBuilder optionsBuilder)
        {
            if (!optionsBuilder.IsConfigured)
            {
                optionsBuilder.UseSqlServer("Data Source=.\\sqlexpress;Initial Catalog=MyOrders;Persist Security Info=True;User ID=sa;Password=***4n**user");
            }
        }

the code will work well.

but if I use a connect string in App.config:

protected override void OnConfiguring(DbContextOptionsBuilder optionsBuilder)
    {
        if (!optionsBuilder.IsConfigured)
        {
            optionsBuilder.UseSqlServer(ConfigurationManager.AppSettings["DataBase"].ToString().Trim());                
        }
    }

it will not work.

the connect string in App.config:

    <add key="DataBase" value="Data Source=.\\sqlexpress;Initial Catalog=MyOrders;Persist Security Info=True;User ID=sa;Password=pk94newuser"/>

the error message:

Instance failure.

the StrackTrace:

at Microsoft.Data.SqlClient.TdsParser.Connect(ServerInfo serverInfo, SqlInternalConnectionTds connHandler, Boolean ignoreSniOpenTimeout, Int64 timerExpire, Boolean encrypt, Boolean trustServerCert, Boolean integratedSecurity, Boolean withFailover, SqlAuthenticationMethod authType)
   at Microsoft.Data.SqlClient.SqlInternalConnectionTds.AttemptOneLogin(ServerInfo serverInfo, String newPassword, SecureString newSecurePassword, Boolean ignoreSniOpenTimeout, TimeoutTimer timeout, Boolean withFailover)
   at Microsoft.Data.SqlClient.SqlInternalConnectionTds.LoginNoFailover(ServerInfo serverInfo, String newPassword, SecureString newSecurePassword, Boolean redirectedUserInstance, SqlConnectionString connectionOptions, SqlCredential credential, TimeoutTimer timeout)
   at Microsoft.Data.SqlClient.SqlInternalConnectionTds.OpenLoginEnlist(TimeoutTimer timeout, SqlConnectionString connectionOptions, SqlCredential credential, String newPassword, SecureString newSecurePassword, Boolean redirectedUserInstance)
   at Microsoft.Data.SqlClient.SqlInternalConnectionTds..ctor(DbConnectionPoolIdentity identity, SqlConnectionString connectionOptions, SqlCredential credential, Object providerInfo, String newPassword, SecureString newSecurePassword, Boolean redirectedUserInstance, SqlConnectionString userConnectionOptions, SessionData reconnectSessionData, Boolean applyTransientFaultHandling, String accessToken, DbConnectionPool pool)
   at Microsoft.Data.SqlClient.SqlConnectionFactory.CreateConnection(DbConnectionOptions options, DbConnectionPoolKey poolKey, Object poolGroupProviderInfo, DbConnectionPool pool, DbConnection owningConnection, DbConnectionOptions userOptions)
   at Microsoft.Data.ProviderBase.DbConnectionFactory.CreatePooledConnection(DbConnectionPool pool, DbConnection owningObject, DbConnectionOptions options, DbConnectionPoolKey poolKey, DbConnectionOptions userOptions)
   at Microsoft.Data.ProviderBase.DbConnectionPool.CreateObject(DbConnection owningObject, DbConnectionOptions userOptions, DbConnectionInternal oldConnection)
   at Microsoft.Data.ProviderBase.DbConnectionPool.UserCreateRequest(DbConnection owningObject, DbConnectionOptions userOptions, DbConnectionInternal oldConnection)
   at Microsoft.Data.ProviderBase.DbConnectionPool.TryGetConnection(DbConnection owningObject, UInt32 waitForMultipleObjectsTimeout, Boolean allowCreate, Boolean onlyOneCheckConnection, DbConnectionOptions userOptions, DbConnectionInternal& connection)
   at Microsoft.Data.ProviderBase.DbConnectionPool.TryGetConnection(DbConnection owningObject, TaskCompletionSource`1 retry, DbConnectionOptions userOptions, DbConnectionInternal& connection)
   at Microsoft.Data.ProviderBase.DbConnectionFactory.TryGetConnection(DbConnection owningConnection, TaskCompletionSource`1 retry, DbConnectionOptions userOptions, DbConnectionInternal oldConnection, DbConnectionInternal& connection)
   at Microsoft.Data.ProviderBase.DbConnectionInternal.TryOpenConnectionInternal(DbConnection outerConnection, DbConnectionFactory connectionFactory, TaskCompletionSource`1 retry, DbConnectionOptions userOptions)
   at Microsoft.Data.ProviderBase.DbConnectionClosed.TryOpenConnection(DbConnection outerConnection, DbConnectionFactory connectionFactory, TaskCompletionSource`1 retry, DbConnectionOptions userOptions)
   at Microsoft.Data.SqlClient.SqlConnection.TryOpen(TaskCompletionSource`1 retry, SqlConnectionOverrides overrides)
   at Microsoft.Data.SqlClient.SqlConnection.Open(SqlConnectionOverrides overrides)
   at Microsoft.Data.SqlClient.SqlConnection.Open()
   at Microsoft.EntityFrameworkCore.SqlServer.Storage.Internal.SqlServerConnection.OpenDbConnection(Boolean errorsExpected)
   at Microsoft.EntityFrameworkCore.Storage.RelationalConnection.OpenInternal(Boolean errorsExpected)
   at Microsoft.EntityFrameworkCore.Storage.RelationalConnection.Open(Boolean errorsExpected)
   at Microsoft.EntityFrameworkCore.Storage.RelationalCommand.ExecuteReader(RelationalCommandParameterObject parameterObject)
   at Microsoft.EntityFrameworkCore.Query.Internal.SingleQueryingEnumerable`1.Enumerator.InitializeReader(Enumerator enumerator)
   at Microsoft.EntityFrameworkCore.Query.Internal.SingleQueryingEnumerable`1.Enumerator.<>c.<MoveNext>b__19_0(DbContext _, Enumerator enumerator)
   at Microsoft.EntityFrameworkCore.SqlServer.Storage.Internal.SqlServerExecutionStrategy.Execute[TState,TResult](TState state, Func`3 operation, Func`3 verifySucceeded)
   at Microsoft.EntityFrameworkCore.Query.Internal.SingleQueryingEnumerable`1.Enumerator.MoveNext()
   at System.Linq.Enumerable.TryGetSingle[TSource](IEnumerable`1 source, Boolean& found)
   at Microsoft.EntityFrameworkCore.Query.Internal.QueryCompiler.Execute[TResult](Expression query)
   at Microsoft.EntityFrameworkCore.Query.Internal.EntityQueryProvider.Execute[TResult](Expression expression)
   at System.Linq.Queryable.FirstOrDefault[TSource](IQueryable`1 source)

I want to know how to use a connect string in EF 6 correctly. Thanks!