'BizTalk WCF service issue

We have exposed orchestration as web service, but sometime caller system getting error as "Request timeout" even if there is no load on BizTalk server.

whenever client send request to BizTalk WCF service hosted in IIS, IIS received it within time ,but message is getting delayed to publish to message box in BizTalk

for example: IIS receives messages at 01:34:45:10, but BizTalk receive pipeline received it at 01:35:15:09 so there is delay of approximate 30 sec between message received in IIS and BizTalk start processing it

This is intermediate issues and happening even if there is no load on server.

Can anyone help me to find out what will be root causes for this



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