'Access ELB from inside the VPC using domain name and subdomain without going to outside internet

How can I access a subdomain from inside VPC going thru the ELB. Let's say have 3 EC2 instances, two of them are running an endpoint API that go thru the Load Balance, and the third one is accessing their API. I have a domain added to Route 53 that has a subdomain for the API endpoint which I am trying to access from within the VPC, is this possible?
What I am trying to do: EC2-3 -> api.mysite.com (stay inside VPC) -> LoadBalancer -> Target group -> either instance 1 or 2



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